Tushar Arora

4 Questions 9 Answers 0 Followers

Questions related from Tushar Arora

What would be the best parameter to study a rising signal (say Calcium signals in live cells) ?

I am stimulating my cultured cells and recording calcium activity as fluorescence change using live-cell calcium imaging. Though I am more concerned about the decay of the signals, for which I am...

10 October 2019 7,768 7 View

I am trying to fit Calcium intensity decay curves on an exponential model - What R-Square value is considered a good estimate of the curve fitting?

I am stimulating my cells in culture using a variety of stimuli and checking the response as Calcium Intensity changes, using live cell calcium imaging. The rise is instantaneous but decay seems...

09 September 2018 3,871 6 View

What would be the best technique to check nuclear translocation of a transcription factor in response to treatment in cell lines ?

I just wanted to check the nuclear translocation of a transcription factor in response to my drug treatment in cell lines ? What would be the best technique to study this ?

09 September 2016 948 5 View

Why have my cells gotten detached & started floating after 4% PFA fixation?

I just started my staining/ imaging studies on HT22 cell line,For that I was fixing the cells after the drug treatment.As soon as I added 4%PFA soln, to the 8 well chamber slide, the cells got...

09 September 2014 9,990 5 View