Tushar Warrier

3 Questions 5 Answers 0 Followers

Questions related from Tushar Warrier

How can I get optimal libraries or what conditions can I tweak to maximise my library concentration for low enrichment ChIP samples??

A lot of my current work involves preparation of ChIP-Seq libraries for a weakly bound factor i.e. a histone modifier in mouse ES cells. Now Im fairly well-versed with ChIP, its troubleshooting...

15 February 2017 6,208 1 View

I wish to confirm whether or not the bioanalyzer traces obtained for the dsDNA (cDNA) for RNA-Seq libraries are ideal? Why is there a trailing peak?

Upon using the TruSeq Stranded mRNA LT kit for RNA-Seq sample preparation I obtain the following Bioanalyzer traces using a Agilent DNA 100 Series II kit. What is the additional trailing peak near...

30 October 2016 8,186 7 View

What would be the most ideal method for expressing and characterizing a transmembrane protein such as an efflux pump in E.coli?

In terms of the expression and characterization of efflux pump proteins, there seem to be several setbacks due to their trans-membrane nature. One method that I know of is the denaturation of the...

18 September 2014 6,921 2 View