Tushar Arora

4 Questions 10 Answers 0 Followers

Questions related from Tushar Arora

What would be the best parameter to study a rising signal (say Calcium signals in live cells) ?

I am stimulating my cultured cells and recording calcium activity as fluorescence change using live-cell calcium imaging. Though I am more concerned about the decay of the signals, for which I am...

24 October 2019 3,982 6 View

I am trying to fit Calcium intensity decay curves on an exponential model - What R-Square value is considered a good estimate of the curve fitting?

I am stimulating my cells in culture using a variety of stimuli and checking the response as Calcium Intensity changes, using live cell calcium imaging. The rise is instantaneous but decay seems...

14 September 2018 6,566 5 View

Is there a way to Convert a drug dose in mg/kg for treating animals to mg/ml for treating cell lines ?

The dose is standardised in animals in mg/kg.i just wanna extend the same treatment to cell lines and was trying to find a way to convert Drug dose from mg/kg to mg/ml ?

23 January 2016 1,184 5 View

Why have my cells gotten detached & started floating after 4% PFA fixation?

I just started my staining/ imaging studies on HT22 cell line,For that I was fixing the cells after the drug treatment.As soon as I added 4%PFA soln, to the 8 well chamber slide, the cells got...

18 September 2014 9,614 4 View