Tuo Yang

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Questions related from Tuo Yang

Does anyone have the experience to perform mouse primary brain endothelial cell isolation and cultivation using Miltenyi microbeads?

I need help with primary mouse brain endothelial cell isolation and cultivation. My cells do not attach to the tissue culture plate (coated with fibronection) after overnight incubation. They are...

08 December 2021 8,111 1 View

How to mimic long-lasting, chronic ischemia (not transient) in primary neuronal cultures with CoCl2?

We would like to establish an in vitro chronic, long-lasting ischemia model in primary (mouse or rat) neuronal cultures with CoCl2. Literatures suggest 1 uM CoCl2 for 7 days detain OPC...

28 March 2018 6,348 2 View