Smeared DNA bands in polyacrylamide gels, but not in agarose gel. Possible reasons?

Hi everyone, I ran some DNAs and saw that they looked normal on an agarose gel but very smeared on a polyarylamide gel. They smeared towards high molecualar weight band. I tried 0.5X or 0.25X TBE...

21 November 2016 3,481 11 View