Tuan Nguyen

5 Questions 2 Answers 0 Followers

Questions related from Tuan Nguyen

What is the most highthroughput in vitro nucleosome binding assay available?

I'm thinking EMSA and streptavidin-magnetic nucleosome pulldown coupled with Western. I don't think these are necessarily highthroughput but they're feasible to do if the constructs are available....

08 August 2018 9,073 1 View

Practical difference between sonication vs french press?

I've worked in both labs that used sonicaiton vs french press to lyse cells. The lab im currently in doesn't have a french press, and some protocols noted the use of french press. I was wondering...

08 August 2018 5,301 1 View

What is the purpose of M9 media in the context of protein expression?

I used to work with M9 minimal media way back for E coli protein expression, but I didn't recall the rationale. Couple years later I read several papers using M9 media for protein expression, I...

08 August 2018 2,765 3 View

Is theere a public database on CHIP-seq and MNase seq?

I'm looking genomewide to see where transcription factor binding sites are enriched for nucleosomes. Thus, I'm looking for the raw genomic datasets where CHIPset, is there a public database...

07 July 2018 8,593 3 View

Advantages/disadvantages of Gibson assembly compared to traditional cloning?

I'm considering giving Gibson assembly a try. I'd like to know what are the potential advantages/disadvantages offered by this method compared to the traditional cloning methods, and what are the...

04 April 2018 6,649 5 View