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Hi all, I was wondering what is the best way to get rid of the organic dye off my mini-gelfiltration column. I washed my column with excess 20X H2O and the dye still seem to be there....
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I have a set of a couple dozen DNA constructs that I plan to order for nucleosome reconstitutions, I was wondering what are the pros and cons of having them as fragments vs cloned. I don't like...
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Does anyone have experience purifying nucleosomes or high molecular complex proteins from gel? I have a small aliquot of nucleosome (180kd+) that I would like to separate from DNA (
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