Can I actually induce memory of T cell in PBMC by doing primary stimulation, resting and restimulation?

Hello, I am new to Immunology. I tried to induce the memory of T cell in PBMC(10%human serum in RPMI) by doing primary antigenic stimulation for 48hrs or 72hrs, and then rest them for 2,4, 6 days...

12 December 2017 1,765 10 View