Troncey Songer

4 Questions 2 Answers 0 Followers

Questions related from Troncey Songer

Why is there no clear interphase during Trizol RNA extraction of fibroblast cells?

It looks almost as if the interphase is floating up into the aqueous phase during my extraction procedure. It doesn't form a distinct layer; it is very irregularly shaped and juts out into the...

02 January 2020 4,770 4 View

Reliability of Gel or Nanodrop ratios for RNA integrity and quality?

I have read somewhere that these should be only taken as a frame of reference? I am assuming that my samples with a 260/280 ratio of ~1.5 are too low, but when I run it on a 1% agarose gel, the...

26 December 2019 3,688 3 View

Can someone help me interpret this RNA gel?

I am trying to determine the integrity for RNA-seq. I just ran a normal 1% agarose gel 60V for 1h. The 28S band seems to be a smear for all the samples, even the ones with a good 260/280 ratio of...

12 December 2019 1,662 2 View

Reliability of Gel or Nanodrop ratios for RNA integrity and quality?

I have read somewhere that these should be only taken as a frame of reference? I am assuming that my samples with a 260/280 ratio of ~1.5 are too low, but when I run it on a 1% agarose gel, the...

12 December 2019 4,675 3 View