Tri Do

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Questions related from Tri Do

Can LB medium contain lactose and lead to leaking of protein expression when before being induced by lactose or IPTG?

I have read an online article saying that there is lactose presence in the LB, which is one of the reasons for leaking protein expression. However, there is no scientific citation in that article...

05 May 2019 543 6 View

Will the precipitated protein at the isoelectric point be dissolved again when the pH of solution is changed?

My project is purification protein and when I change solution by dialysis, my protein is precipitated at the time pH of solution is pI. So how to protein can dissolve again? Thanks you!

12 December 2018 6,352 4 View

Can LB medium contain lactose and lead to leaking of protein expression when before being induced by lactose or IPTG?

I have read an online article saying that there is lactose presence in the LB, which is one of the reasons for leaking protein expression. However, there is no scientific citation in that article...

01 January 1970 614 3 View