Tong Gao

3 Questions 8 Answers 0 Followers

Questions related from Tong Gao

Does anyone have any information about over dose of IL-4 expression in mice?

I injected 4X10E12 AAV-IL4 in mice via iv injection, 5 Days later, mice die. The control mice were fine up to 5X10E13. Is that because of side effection of IL4 overexpression?

02 February 2015 7,541 5 View

Anyone knows how to concentrate condition medium from AAV triple transfected 293 cells growth media?

Currently I am working on AAV triple transfection. According to the paper Lock,etal, human gene therapy, 2010. AAV virus can release from cells into media. We do not have TFF as what they used in...

12 December 2014 6,146 19 View

Does anyone knows why IGF-1 treated splenocytes died after 48 hours treatment?

Currently I'm working on IGF-1 activity on T-cell marker experssion. I isolated mouse splenocytes and treated cells with 100 ng/ml IGF-1, splenocytes were cultured in RPMI 1640 plus 10% FBS,...

01 January 1970 169 2 View