Tong Gao

3 Questions 3 Answers 0 Followers

Questions related from Tong Gao

What the concentration of ionomycin and PMA used for splenocytes stimulation?

I'm working on IGF-1 effects on cytokine expression in CD4+ cells. According to BioLegend reference, I used 500ng/ml ionomycin and 500ng/ml PMA to stimulate splenocytes as well as GolgiStop (BD...

31 August 2016 3,853 5 View

Does anyone have any information about over dose of IL-4 expression in mice?

I injected 4X10E12 AAV-IL4 in mice via iv injection, 5 Days later, mice die. The control mice were fine up to 5X10E13. Is that because of side effection of IL4 overexpression?

25 February 2015 7,562 4 View

Does anyone knows why IGF-1 treated splenocytes died after 48 hours treatment?

Currently I'm working on IGF-1 activity on T-cell marker experssion. I isolated mouse splenocytes and treated cells with 100 ng/ml IGF-1, splenocytes were cultured in RPMI 1640 plus 10% FBS,...

01 January 1970 5,879 2 View