What is the best linkers for 3xHA tag? Is linker(s) required between tags and/or between tag and an gene of interest?

I am trying to make 3xHA tagged-gene construct. I have found some constructs with 3xHA on Addgene and the 3xHA tags have several linkers between HA tags. Some have G and GS between HAs, but others...

24 July 2018 3,974 6 View