Tomohiro Suzuki

2 Questions 2 Answers 0 Followers

Questions related from Tomohiro Suzuki

Does anyone know why false positive colonies of E. coli are obtained after transformation?

I am trying to construct a Piggybac retrotransposon vector to stably express my target gene in a mammalian cell line. However, I have a big problem: only false positive colonies are obtained after...

02 August 2018 864 5 View

Does anyone know a good tool to predict the off-target effect of an already designed gRNA?

I am trying to find a good negative control gRNA in my experimental system. I found that Zhang laboratory provides sequences of non-targeting gRNA in their gRNA libraries, some of which are used...

22 May 2018 4,504 1 View