Tommy Lo

3 Questions 5 Answers 0 Followers

Questions related from Tommy Lo

Can anyone share a general protocol of Western blot using Urea/Thiourea/CHAPS lysis buffer?

Hello all, I have only done few rounds of western blots using a standard protocol with RIPA. I would like to try to use another buffer 8M Urea2%CHAPS2M Thiourea40mM DTT1% NP-40water I am a bit...

02 February 2016 4,634 2 View

What is the solution to low viability of mouse ESC upon passaging? (Feeder-free)

Hello everyone, I have started to culture mouse embryonic stem cells (mESC) recently. The mESC lines are produced by one of my labmates and are verified by teratoma assay and other means. Then my...

12 December 2015 2,391 6 View

How can a mouse splenocyte be cultured?

I have isolated some mouse splenocytes by meshing the spleens through cell strainers (70um). However due to bad timing I have to wait for at least 2 days for the reagents to come. I wonder if I...

03 March 2014 8,874 4 View