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Hello all, I have only done few rounds of western blots using a standard protocol with RIPA. I would like to try to use another buffer 8M Urea2%CHAPS2M Thiourea40mM DTT1% NP-40water I am a bit...
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I have just isolated some tail tip fibroblasts from very young (1-wk old) mice for experimentation. I am using IHC to confirm the identity of the fibroblasts. I have looked at a few Q&A topics...
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I have isolated some mouse splenocytes by meshing the spleens through cell strainers (70um). However due to bad timing I have to wait for at least 2 days for the reagents to come. I wonder if I...
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