Multiple bands in SDS PAGE after induction with IPTG of GST-protein of interest?

I am using E. Coli B21 as a vector with pGEX-2TK plasmid. I induced my fusion protein with IPTG, creating GST-protein of interest. I loaded an SDS-PAGE gel: in the first well, I loaded total...

30 March 2019 5,286 2 View