Tomáš Hajkovský

4 Questions 7 Answers 0 Followers

Questions related from Tomáš Hajkovský

Any advice or new protocol about preparation of electrocompetent E. coli BL21 (DE3) Rosetta cells with highest electrocompetent efficiency possible?

Hello, my electrocompetent E.coli BL21 (DE3) cells are having low transformation efficiency with pAC28 vector-insert (10 - 30 kb). It was around 1 × 10^4 and less / 1microgram of...

19 April 2016 8,910 5 View

Do you know soil DNA isolation kit that isolates DNA 50-100 kb long?

Hello, I looked through some soil isolation kits but most of them use also mechanical lysis of cells. Do you know about any soil DNA isolation kit giving HMW DNA 50-100 kb long? For example...

30 December 2015 5,563 3 View

What is the lowest threshold of gravitational acceleration [g, RCF] for centrifuging high molecular mass DNA?

I have purified and isolated 50 - 100 kb and 120 - 200 kb DNA fragments from metagenome. I work with them separately. I know that so large fragments tend to shear if centrifuged. But is there at...

15 December 2015 9,712 5 View

Do I have DNAse contamination of metagenomic DNA sample or is something else (PFGE Electrophoretogram attached)?

Hello everybody, please, do you have some smart advice to solve this problem? Here are two electrophoretograms from pulse field gel electrophoresis. In both experiments metagenomic DNA was being...

10 November 2015 5,615 2 View