Tom Jonesman

7 Questions 11 Answers 0 Followers

Questions related from Tom Jonesman

Which outgroup and which genes are used for angiosperm phylogeny?

I need to construct a phylogenetic tree for 4 grasses (Festuca, Holcus, Anthoxanthum and Agrostis) and 4 forbs (Leucanthemum, Centaurea, Rumex and Plantago), but have little experience in plant...

29 November 2013 5,476 2 View

Is there a way to return upper and lower values of a simulated envelope?

The attached figure is a mark correlation function produced by the spatstat package in R. The summary returned from the object used to produce this figure is as follows: r theo...

01 November 2013 7,340 3 View

What is the best way to measure percentage root colonization by Arbuscular Mycorrhizal fungi?

I have so far read McGonigle et al. 1990: "A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi", as I am preparing to collect plant...

11 July 2013 6,646 3 View

Generating Rank abundance curves in R?

I'm using the BiodiversityR package to generate rank abundance curves. The following code scales the y axis as proportional abundance (species abundance/total abundance). The y axis then...

18 June 2013 1,463 4 View

What do you use to incubate restriction digestion reactions?

I have been using a Hybaid Shake n' stack style incubator (http://www.fishersci.com.sg/product_details.asp?nid=761), but was wondering if anybody uses a water bath instead, perhaps for improved...

11 June 2013 8,119 43 View

Does anybody use the primers AML1 and AML2 instead of AM1 and NS31 in arbuscular mycorrhizal fungal research?

Instead of the universal eukaryotic primer NS31 and the AM-specific primer AM1, I am wondering if anyone has used the primers developed by Lee et al (2008) that amplify a much higher proportion of...

05 June 2013 9,220 5 View

How does SYBR safe compare with Ethidium bromide?

There are fewer health hazards associated with SYBR safe, but based only on results, how does SYBR safe compare with ethidium bromide? Does it intercalate more effectively with DNA or fluoresce to...

18 April 2013 6,930 9 View