Tobias Benjamin Schuster

7 Questions 6 Answers 0 Followers

Questions related from Tobias Benjamin Schuster

What is the best way to carry out preparation of a cholesterol solution?

What is the best way to dissolve cholesterol to check the interaction with recombinant proteins in vitro and with proteins in cell lysate?

23 May 2014 3,487 5 View

Any suggestions for electroporation parameters using Biorad Gene Pulser Xcell to transfect mouse melanoma cells B16-F0?

Electroporation of mouse melanoma cell line B16

10 July 2013 3,243 0 View

Any experience with in vitro translation of disordered proteins using mammalian lysate?

I would like to use rabbit reticulocyte lysate to translate different disordered proteins from mRNA.

28 March 2013 2,357 0 View

Less mass spectroscopy protein identifications of in-gel digestion compared to in-solution digestion?

I would like to identify interactome of my bait protein via immunoprecipitation with an orbitrap instrument. Do you recommend in-gel solution to get rid of detergents? Is it likely to loose...

17 March 2013 8,663 5 View

How do you analyze secretion of a protein expressed in in vitro tranlation with rabbit reticulocyte lysate?

What proteins can I use as positive control for secretion?

17 March 2013 606 0 View

Is using a rabbit primary antibody in rabbit reticulocyte lysate a problem?

Are there possible cross reactions or background using rabbit primary antibodies to detect in vitro translated human proteins from mRNA in rabbit reticulocyte lysate?

09 March 2013 8,598 0 View

Does anyone know a good tutorial for using R in mass spec data sets?

I want to manage and analyse my data sets from mass spec experiments with the program R. I'm just a beginner in R, so I would be happy for any suggestions.

28 October 2012 4,103 3 View