Tobias Benjamin Schuster

9 Questions 6 Answers 0 Followers

Questions related from Tobias Benjamin Schuster

What is the best way to carry out preparation of a cholesterol solution?

What is the best way to dissolve cholesterol to check the interaction with recombinant proteins in vitro and with proteins in cell lysate?

23 May 2014 3,526 5 View

What is the best method to purify BAC for transfection?

It may not be easy to transfect such big DNA constructs of several hundred kb into mammalian cells.

13 March 2014 4,927 1 View

Might sodium acetate in purified plasmid DNA affect transfection efficiency of mammalian cell lines?

After using plasmid prep you can concentrate DNA using 3M sodium acetate buffer solution. Maybe there remains acetate in the DNA solution?

27 February 2014 6,024 0 View

Any suggestions for electroporation parameters using Biorad Gene Pulser Xcell to transfect mouse melanoma cells B16-F0?

Electroporation of mouse melanoma cell line B16

10 July 2013 3,279 0 View

Any experience with in vitro translation of disordered proteins using mammalian lysate?

I would like to use rabbit reticulocyte lysate to translate different disordered proteins from mRNA.

28 March 2013 2,394 0 View

Less mass spectroscopy protein identifications of in-gel digestion compared to in-solution digestion?

I would like to identify interactome of my bait protein via immunoprecipitation with an orbitrap instrument. Do you recommend in-gel solution to get rid of detergents? Is it likely to loose...

17 March 2013 8,702 5 View

How do you analyze secretion of a protein expressed in in vitro tranlation with rabbit reticulocyte lysate?

What proteins can I use as positive control for secretion?

17 March 2013 646 0 View

Is using a rabbit primary antibody in rabbit reticulocyte lysate a problem?

Are there possible cross reactions or background using rabbit primary antibodies to detect in vitro translated human proteins from mRNA in rabbit reticulocyte lysate?

09 March 2013 8,632 0 View

Does anyone know a good tutorial for using R in mass spec data sets?

I want to manage and analyse my data sets from mass spec experiments with the program R. I'm just a beginner in R, so I would be happy for any suggestions.

28 October 2012 4,152 3 View