Tobias Haar

3 Questions 1 Answers 0 Followers

Questions related from Tobias Haar

How to calculate p-values from CT values for RT-qPCR data?

Input: - RT-qPCR dataset row CT values interpolate corrected - 50 target genes - 10 reference genes - 3 different treatments with 3 replicates each - 1 control replicate for each...

05 September 2019 1,855 3 View

How to deal with non-detects in CT data when performing Delta Delta CT Method?

Input: - RT QPCR dataset row CT values interpolate corrected - 50 target genes - 10 reference genes - 3 different treatments with 3 replicates each - 1 control with 3 replicates Objective: -...

20 August 2019 1,679 3 View

How to deal with multiple ensemble IDs mapping to one gene symbol in a RNA-Seq dataset?

Input: - Normalized dataset based on RNA-Seq with ensemble IDs as row names. Problem: - Multiple ensemble gene IDs are mapping to the same gene symbol. Thus the same gene symbol has multiple...

20 August 2019 9,725 11 View