Why am I observing a band at 20kb after ligating a 1.5kb insert with a 4.4kb pCold I plasmid?

I am observing a band at 20kb after ligating a 1.5kb insert with a 4.4kb pCold I plasmid, which should ideally result in a band around 5.9kb (assuming successful ligation). I used BamHI-HF (NEB)...

21 May 2024 9,605 3 View