Tiwa Ogunleye

2 Questions 5 Answers 0 Followers

Questions related from Tiwa Ogunleye

What is a good lysis buffer detergent for membrane protein purification?

Does anyone have an idea of a good lysis buffer detergent for membrane protein purification? I am working on purifying a single-span membrane protein, and I have tried SDS, IGEPAL, Triton, and...

21 April 2022 5,545 4 View

What is a good buffer to dialyze membrane proteins post Ni-nta affinity purification?

I have been using just di-H2O with little to no recovery.

03 September 2021 9,423 5 View