Tingyi Li

2 Questions 2 Answers 0 Followers

Questions related from Tingyi Li

How to optimize vector backbone to have higher plasmid yields?

Hi, I am trying to optimize plasmid yield to prepare for animal experiments. So far, I can only get about 0.6~0.7mg from 1L starting culture. I used the Zymo Max prep kit, and I don't think that...

01 November 2019 7,916 2 View

Can someone advise on RT-qPCR, high Ct values on GAPDH?

I am working with HUVEC cells, and tried to plate 7500, 10000,15000,and 20000 cells on 48-well plate to see how many cells are good enough for RT-qPCR. I isolated RNA using Trizol/Qiazol method,...

20 April 2018 9,415 4 View