Can anyone suggest how many ul of S-adenosyl-methionine (SAM) are required in optimizing restriction endonuclease digestion with PCR product?

I would like to understand what volume of SAM is ideal for RFLP using the recommendation for use of the methylation reaction where the SAM should be diluted 1:400 to a final concentration of 80μM.

20 May 2014 6,215 6 View