Tian Jiang

7 Questions 5 Answers 0 Followers

Questions related from Tian Jiang

Is there any methods to culutre the primary pancreatic acinar cell? (from mouse)?

I am working on pancreatic acinar cell function. And I am eager to find out how to do acinar cell related experiments in vitro. I have tried some methods to generate pancreatic organoids wishing...

05 December 2024 113 4 View

Pancreatic Acinar cell line?

I am working on pancreatic acinar cell function. I have checked so many papers it seems there is no acinar cell lines or organoids so far. I am wondering like-minded researchers who work on...

07 October 2024 8,939 3 View

The components of a pancreatic exocrine organoid using mice pancreas?

I am working on pancreatic cancer. Now, I am trying to generate a pancreatic exocrine organoids using my KPC mice. But after I checking the protocol, PancreaCult™ Pancreatic Organoid Growth Medium...

21 April 2024 3,941 1 View

What is the best timing to inject tamoxifen to induce PDAC from KPC mouse?

I really want to know what is the best timing to inject tamoxifen to induce PDAC. My preliminary experiments is not enough to give a suggestion. I have read some papers and they have different...

05 December 2023 9,312 0 View

Insert two DNA fragments into one vector at the same time, is it possible?

My colleague has come up an idea of using two inserts to ligate into one vector at the same time, which is like : vector-SpeI-Insert A-BamHI-Insert B-NotI-vector (Spel-Insert A-BamHI, BamHI-Insert...

29 November 2023 900 2 View

What is solution to insert fragments into single restriction enzyme cutting sites BamHI?

I selected a single restriction enzyme cutting site BamHI in a plasmid of around 6 kbp, performed enzyme digestion, and carried out dephosphorylation treatment. I attempted to insert a fragment of...

26 November 2023 1,198 1 View

Insert two DNA fragments into one vector at the same time, is it possible?

My colleague has come up an idea of using two inserts to ligate into one vector at the same time, which is like : vector-SpeI-Insert A-BamHI-Insert B-NotI-vector (Spel-Insert A-BamHI,...

01 January 1970 6,813 2 View