Does anyone use a homemade master mix with SYBR green I to do qPCR on RotorGene Q?

I have a RotorGene Q (Qiagen) and I'll perform qPCR reaction for relative quantification. I have SYBR green I 10.000x and the other components of the reaction. Can I make a homemade master mix?...

21 May 2019 10,041 3 View