Thupten Tsering

2 Questions 8 Answers 0 Followers

Questions related from Thupten Tsering

Has anyone used Topoisomerase I to relax the supercoiled DNA, (PhiX 174RF I DNA)?

I want to know the composition of 10X reaction buffer and how does it look in agarose gel picture after using Topoisomerase I. Does it have multiple bands or single band ? Using Topoisomerase I on...

12 March 2015 9,780 9 View

My DNA dialysis against MilliQ results in lower purity and concentration (ng). Can you help me with this problem?

Hi, I have been doing DNA plasmid PhiX 174 RF I DNA dialysis against milliq water and purity after dialysis is 1.6. Before Dialysis i have checked and it was 1.92. For my further experiment I...

03 March 2015 4,853 4 View