Thomas Cafiero

2 Questions 2 Answers 0 Followers

Questions related from Thomas Cafiero

What is the best gel filtration media to remove contaminants from my purified protein ?

We are working on purifying a 60 kDa and have been having issues ridding the fractions of lower MW protein contaminants. We've previously used Ni-NTA and Ni Sepharose 6 fast flow resin for...

03 March 2019 8,242 5 View

Why am I losing all of my target protein during purification ?

I have been attempting to purify a His-tagged bacterial chaperonin protein. I have had no issue over-expressing the protein; however, it appears my protein is not binding well enough to my column...

01 January 2019 4,018 8 View