Thomas Heida

5 Questions 3 Answers 0 Followers

Questions related from Thomas Heida

Why linear increase of eGFP expression with RTS 100 E.coli kit?

Having not so much experience with cell-free expression systems, I do have a general question. I am using a commercial RTS 100 E.coli kit to express eGFP. When following the expression on a tecan...

26 January 2019 2,302 3 View

How to avoid hydrogel particle sticking on plastic vials?

I am working on biofunctional (DNA-modified) hydrogel particles, which need to be washed by centrifugation. When performing in water, particles completely sedimentate. However, when washing with...

09 January 2019 8,827 1 View

How can I avoid DNA-aggregation in hyaluronic acid solution?

As a chemist, I am working on hyaluronic acid (HA) hydrogels, which are DNA-modified. DNA is attached to HA via Thiol-Michael. However, due to the polyanionic character of HA, DNA forms aggregates...

20 November 2018 2,511 3 View

Dissolution of Maleimide-Neutravidin in MES-Buffer pH 5.5. How to prevent aggregates ?

Hello, as a chemist, I'm currently working on the covalent conjugation of Maleimide-Neutravidin to furan-modified hyaluronic acid as precursor material for microbead preparation. To perform...

19 September 2018 1,605 2 View

Side reaction between thiol groups and MES Buffer?

Hello everyone, I am working on the thiol functionalization of hyaluronic acid. Is there in general any side-reaction going on between MES-buffer and free thiol groups when i am dialysing my...

07 December 2016 5,126 3 View