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I am trying to use overlap extension PCR to combine 2 linear PCR fragments around 1kb each. I amplified both fragments with overhanging primers with a 20 bp overlap between the two fragments. When...
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I am getting 3PCR products of different sizes when amplifying off a plasmid, and I think it is due to unintentional primer binding. I am amplifying a trimer off the plasmid, and the 3 sizes...
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