Thomas Byrne

3 Questions 2 Answers 0 Followers

Questions related from Thomas Byrne

Does PHA-L stimulate the proliferation of magnetically isolated T cells?

Hello, I want to measure the proliferation of magnetically isolated T cells. I have successfully stimulated PBMC with 5 ug/mL PHA-L and measured their proliferation with MTT. The same method has...

29 October 2020 7,234 3 View

How do I scale up my PBMC isolation from 15 mL tubes to 50 mL tubes?

In our lab, we isolate PBMC from undiluted whole blood using Histopaque. This works perfectly when layering 3mL undiluted whole blood over 3 mL Histopaque. The problem is when working with larger...

08 October 2020 1,707 3 View

Hi, I want to analyse the migration of recent thymic emigrants in vitro. Will the under agarose migration assay work for this?

I am looking for an alternative to transwell migration assays as they are too expensive for the number of samples I need to analyse. I just want to know if the under agarose migration assay is...

28 February 2019 1,775 1 View