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Hello, I want to measure the proliferation of magnetically isolated T cells. I have successfully stimulated PBMC with 5 ug/mL PHA-L and measured their proliferation with MTT. The same method has...
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In our lab, we isolate PBMC from undiluted whole blood using Histopaque. This works perfectly when layering 3mL undiluted whole blood over 3 mL Histopaque. The problem is when working with larger...
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