Thiru Sabapathy

3 Questions 6 Answers 0 Followers

Questions related from Thiru Sabapathy

How to maximize immobilization of lipoparticles on sensor chipL1 used in BiacoreT200 (Surface Plasmon Resonance) ?

I am trying to study membrane protein interaction with its ligand in native cell membrane environment. For this, I am using lipoparticles generated from a stable cell line to couple with SPR...

24 June 2016 988 3 View

Can anyone suggest an alternative method for trypsinizing CHO cells?

I am working on CHO cells and am trying to come up with an alternate method for trypsinizing the adhered cells. Since my study is about estimating the activity of insulin receptors, I am afraid...

03 October 2014 8,728 3 View

What are the optimal conditions of ultra-centrifuge to maintain while separating solubilized membrane proteins?

I scraped the cells out of the flasks and homogenized them with mortar and pestle type homogenizer. I solubilized the membrane particles with 1% Triton X100. In order to separate the solubilized...

03 September 2014 9,923 7 View