Thiéry De Serres-Bérard

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Questions related from Thiéry De Serres-Bérard

Why do my brain organoids don't display forebrain identity?

Hi! I am producing forebrain brain organoids using the Lancaster protocol (Lancaster, 2014, Nature protocols). I successfully produced brain organoids with forebrain identity the first time I...

10 November 2020 8,056 2 View

Why does there is a lot of condensation in the lid of petri dish that are used for cell culture in an incubator on a orbital shaker?

I have observed that when I culture cells or organoids on a petri dish that are placed in an incubator (with humidity, 37 C and 5% CO2) on an orbital shaker at 80 rpm, there is more water...

28 August 2019 509 3 View

Why do some hiPSC lines make ellipsoid embryoid bodies instead of spheroid embryoid bodies when using the self-aggregation method?

I am producing embroïd bodies derived from hiPSC to generate brain organoids by the self-aggregating method following the protocole published by Lancaster in 2013 and 2017. Briefly, I seed 9000...

28 August 2019 4,846 3 View