Thierry Michon

2 Questions 16 Answers 0 Followers

Questions related from Thierry Michon

How to perform an in vitro phosphorylation of recombinant proteins?

We study the properties of a complex between two proteins. We have recombinant forms of these proteins purified from E. coli. We suspect that the phosphorylation of one of these proteins...

26 September 2013 5,091 18 View

Native PAGE: is it possible to substitute beta alanine with 6 amino hexanoic acid ?

Here is my usual protocol for native page: Separating gel buffer contained 8% acrylamide (aa:bis30%:0.8%) in 1.5 M acetate-KOH (pH 4.5), plus 70 μl 10% ammonium persulfate (APS) and 2.5 μl...

20 November 2012 2,907 6 View