Theros Ng

3 Questions 9 Answers 0 Followers

Questions related from Theros Ng

Neutrophil ROS assay no cell control has higher fluorescence than DHR stained cell?

In my neutrophil ROS production assay, fluorescence of the no cell control with DHR is higher than the cell stained with DHR? Basically, column 2 is always higher than column 4. Example: Column...

02 February 2019 6,265 2 View

How long does it take to lyse bacteria using beads?

I want to lyse bacteria using beads for ELISA. Campylobacter to be specific. How long do I have to lyse it for and at what speed and time of centrifugation after?

08 August 2016 7,439 4 View

Why didn't my ELISA for campylobacter IgA in chicken bile work?

I have been running ELISAs for a week but I can't get it to work. I coated the 96well plate with 100ul of 20ug/ml of campylobacter jejuni in 1M bicarbonate buffer overnight at 4C. Washed 3 times...

08 August 2016 2,890 12 View