When I have a DNA:RNA hybrid with approximately 100 bp long DNA and 8 nt long RNA, would a ssDNA strand complementary to the DNA displace the RNA?

I want to design a template for in vitro transcription of RNA Pol II and have a questions regarding the construction of the DNA:RNA hybrid. I have an approximately 80-200 nt long ssDNA (template...

12 August 2025 5,035 2 View