Therese M Becker

3 Questions 7 Answers 0 Followers

Questions related from Therese M Becker

What is/are the most reliable referenece gene(s) for gamma irradiation gene expression experiements?

I am setting up some experiments to detect gene expression changes after gamma irradiation and am after a good housekeeping control gene that is not influenced by the irradiation. Does anybody...

09 September 2014 4,985 3 View

Are there any good non-rabbit, non-mouse anti-human-CD45 antibodies around that work well in immunocytostaining (or FACS)?

I am isolating CTCs with a combination of mouse antibodies and identify them with rabbit antibodies. To make life easier I would like to use a goat (or other host) anti-CD45 antibody to identify...

12 December 2013 8,623 0 View

Does anybody have any experience (reliability, costs) with buffers or kits (made or commercial) to perform cell lysis for direct use in PCR?

We want to use PCR on small cell numbers and avoid loss of DNA by DNA purification procedures.

11 November 2013 8,010 6 View