Tharaka Darshana Wijerathne

10 Questions 33 Answers 0 Followers

Questions related from Tharaka Darshana Wijerathne

Comparing effect of a drug at Different temperatures with unpaired data?

Hi Everyone I have calculated the effect of a drug on the inactivation of a protein at different temperatures using unpaired experiments. The drug has a significant effect (p

12 January 2022 2,206 3 View

Why inside out Single Channel Potassium currents are recorded with a positive holding potential?

Hi :) Im trying to start obtain on Single channel data on Potassium ion channels. So far I have worked with whole cell condition and I have no experience with single channel experiments. There is...

25 September 2017 2,595 7 View

Is it OK to Ground the patch Clamp Head-stage?

Hi all I was having an issue with my patch clamp rig. The membrane test was showing a small current (~100nA) even when the patch pipette was not touching the bath solution. Furthermore there was a...

20 April 2017 887 13 View

Permeability Calculation using Reversal Potential?

Hi I'm trying to calculate the Monovalent cation permeability of TRPC6 channels. I did the experiments and got the results. However, the problem is the values I get for NMDG. NMDG gets about 0.1...

12 December 2016 1,686 2 View

How to Calculate Time constant in BK channel Step Current recordings?

Hi I'm testing an effect of a drug on voltage gated potassium channel Slo3. I can see a clear change in current response to Step Pulses (shown in attached image). The voltage step protocol that...

13 July 2016 8,567 11 View

How to Predict EF hand (Calcium Binding Protein)?

I need to find weather a region of a protein that I'm currently working on has a Calcium Binding Domain. The putative region has a similar amino acid profile as an EF domain. I would like to run...

01 July 2016 4,358 6 View

How do I Calculate/Plot a Conductance-Voltage Curve from Current-Voltage Data?

Hi all I have Current Voltage data obtained from voltage clamp recordings. The data is from HEK293 cells over expressing BK(Slo1) channels. I used a step protocol (holding at -100mV | +24mV steps...

07 December 2015 5,541 18 View

Why are my non-transformed competent cells grow in Ampicillin?

Hi I had did not have any yield with mini & Midi prep in last few trials. I used pCDNA3 vectors, so I used Ampicilin as the selective antibiotic in bacteria culture. I got results one time...

01 June 2015 2,090 10 View

Why is there no DNA after midiprep even though there was enough bacterial growth?

I was trying to multiply three plasmids (DNA of same ion channel from rat, mouse and human in PCDNA3.1) through transformation. There were colonies after transformation and sufficient bacteria...

25 February 2015 4,341 9 View

Patch Clamping| Mobility of BAPTA or a way to calculate the liquid junction potential of solutions contain BAPTA using Clampex software?

I have data obtained from patch-clamping on reversal potential of an ion channel. I need to correct my readings for liquid junction potential. I used Axon instruments for path-clamping and I I...

13 October 2014 9,886 10 View