Thao Tran

2 Questions 2 Answers 0 Followers

Questions related from Thao Tran

Can I block the western blot over the weekend?

I want to block with 5% non-fat dry milk over the weekend? Is possible that it is too much blocking?

06 June 2016 8,770 1 View

I accidently incubated my western blot membrane with Abcam stripping buffer overnight at 4degrees. Is it still work ?

This is protocol that I suppose to follow Buffer, 1 liter 15 g glycine 1 g SDS 10 ml Tween20 Adjust pH to 2.2 Bring volume up to 1 L with ultrapure water. Membrane incubation Use a volume that...

06 June 2016 1,818 3 View