Thanh Nguyen

3 Questions 16 Answers 0 Followers

Questions related from Thanh Nguyen

Can bacterial DNA be extracted by boiling with DI water or can Lysis buffer be used in qPCR?

I am planning on running qPCR but with bacterial DNA. Will the qPCR work with the DNA extracted by boiling culture with DI or lysis buffer? Or I have to extract the genomic DNA using the kit...

30 October 2014 8,768 4 View

Why do the Ct values of qPCR reactions vary?

I have run multiple qPCR on my water and sediment samples. Every time I run, I get different Ct values, which means the number of copies also changes (by a factor of 10). What might have happened?...

20 October 2014 5,676 12 View

How do you extract total DNA from water samples?

I would like to extract total community genomic DNA from water samples. I tried to filter the water with filter papers then extract the DNA off the paper using PowerSoil DNA isolation. The DNA...

24 September 2014 3,654 25 View