Thangadurai Mani

2 Questions 1 Answers 0 Followers

Questions related from Thangadurai Mani

Protocol or kit that you would recommend to extract plasma membrane proteins from mammalian cells?

I am trying to endogenous express MDR1 protein in a mammalian cell and I would like to confirm the presence of the expressed protein in the plasma membrane of different patches of cell populations...

01 January 2014 7,555 1 View

I am trying to grow transfected mammalian cell lines in a 24 well plates but the cells on 4 sides of the wells died completely

I am just wondering what might have caused this as this is happening many number of times and also in 12 wells and 6 wells plates. I know it might be because of edge effect/evaporation but the...

08 August 2013 4,357 5 View