Thang Luong Pham

4 Questions 16 Answers 0 Followers

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What is the best concentration for initiating the ARPE19 / RPE in T-flask and N-well plate with DMEM-F12?

Dear friends, I am working on RPE (human/mice primary RPE and ARPE19) for a while. The seeded density is 100.000 cells/ml. For T-75 flask, 6-well plate I used 20 ml and 3 ml/well respectively. The...

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I am going to conduct ELISA assay, but I do not know what microtitration plate is best for high efficiency of protein fixation.

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What is the best immunologic adjuvant for mice and rabbits?

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Do you have any e-books on cloning guidelines for selecting restriction enzymes when RE sites are located on both the target gene and Multi Cloning Site?

I am a master student and need to learn about choosing the right cloning strategy. Therefore, I really want to read more books about that issue. Any suggestions?

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