Thandiwe Chavula

3 Questions 3 Answers 0 Followers

Questions related from Thandiwe Chavula

How best to design HDR donor template with custom tags for CRISPR?

I am trying to replicate the methods in this paper:Purification of an Intact Human Protein Overexpressed from I... ) to design/order an HDR donor with the following tags: GFP-Hygr, CMV-promoter,...

10 February 2025 3,178 1 View

What is the best source for murine dendritic cells in vivo to study phagocytosis/efferocytosis?

I am studying phagocytosis/efferocytosis in vivo and have been using the peritoneum as a source of cells, but have a very low yield of dendritic cells. I also have a lot of CD19+ B-cells that...

22 September 2020 2,194 3 View

What are the best internal controls to use in Micro-RNA qPCR?

I am working with microRNA in Lupus samples and running qPCRs. I am currently using U6 as the internal control, and wondering if there are better controls to use because my data analysis is not...

01 January 1970 6,079 6 View