Thaíssa Engel

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Questions related from Thaíssa Engel

What is the expected HPLC chomatographic profile for the purification of a DNA oligo conjugated to an ATTO 647 dye?

We have synthesized amino labeled oligos at our lab, and performed post syntetic conjugation to ATTO 647 (NHS), following the original protocol we got from the supplier. We purified the oligo by...

31 March 2025 6,742 3 View

What HPLC column do you use for DNA oligo probes analysis / purification?

I have used C18 columns for standar oligonucleotides, but when it comes to dual-labeled oligos, as for instance a probe with FAM 5' and 3' BHQ1, it is not that straight forward. I mean, when you...

31 January 2024 8,468 0 View

What synthesizer do you use to make DNA / RNA / LNA oligos?

Are you a fellow oligo chemist? What synthesizer do you use to make your oligos? What are the best features of this machine? What are the weak points of it? My goal with this question is getting...

15 January 2024 918 0 View

How to fix slide problems in a MerMade6 (LGC) DNA/RNA oligo synthesizer?

I use a DNA/RNA synthesizer from LGC (Biosearch), a MerMade6, to make oligos. The machine has operated for around 16 months and produced about 1500 oligos during this period. As you can see, it is...

15 January 2024 10,063 7 View