Tetsuya Takahashi

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Questions related from Tetsuya Takahashi

How can we check the expression of Arf6 Q67L?

We are using expression vector pcDNA3 HA Arf6 ActQ67L (Plasmid #10835) obtained from Addgene. But we could not detect the expression of the product through WB using an anti-HA antibody (both...

10 October 2019 2,143 2 View

Can anyone suggest an expression vector that is best to produce PIP2 positive vesicles in culture cells?

I am attempting to produce intracellular PI(4,5)P2 coated vesicles by introducing an expression vector for a constitutive active form of Arf6(Q67L). After that, I would like to observe large...

12 December 2018 3,019 0 View