Kinase, Ligase and Dpnl in Q5 Site-Directed Mutagenesis (NEB) - What is the principle?

Hello, I am reading the Q5 Site-Directed Mutagenesis (NEB) protocol. I understand that the phosphorylation step is needed for subsequent ligation. But I wonder what is the principle of removing...

11 August 2021 6,058 4 View