Terence Spencer Crofts

3 Questions 15 Answers 0 Followers

Questions related from Terence Spencer Crofts

Why are no bubbles produced during catalytic transfer hydrogenation with Pd/C?

I am working on reducing an aromatic nitro group to amine and have tried SnCl2 and Fe systems without much luck. There are literature examples of this compound being reduced by H2 gas with Pd/C...

01 November 2017 3,816 3 View

Do enzymes require high buffer concentrations when stored at -80 C?

I have a project studying the activity of an enzyme in vitro. My assay for activity is run in 5 mM MOPS buffer pH 7.2 and I can't change this. Optimally, after purifying my enzyme from an E. coli...

16 March 2016 9,624 3 View

How well does Pichia pastoris handle exons/introns?

I want to express eukaryotic genes and yeasts seem a good bet to construct a library in. S. cerevisiae can't handle exons or introns, but I have not found definitive information about if Pichia...

11 July 2014 8,822 2 View