Tehmina Saleem Khan

2 Questions 12 Answers 0 Followers

Questions related from Tehmina Saleem Khan

How to do mutation in my gene of an enzyme to check location/amino acid sequence of its active site?

I have amplified and cloned a gene from bacteria. It has good expression in pET28-Rosetta. Moreover, it is soluble and active towards substrate. Now, lets assume I have to check its catalytic...

04 April 2019 6,122 6 View

If a gene has multiple start codons (ATG), it can be amplified and express easily?

I am trying to amplify a gene from streptomyces genome. This is GC rich with 36 start codons (ATG) at different positions of whole gene (1491 bp total gene length). I am not getting the right...

08 August 2018 1,345 3 View