Why lower molecular weight protein bands disappeared in Tricine SDS PAGE?

Hi, I run this gel using 3 different layers: -the upper layer: 4%T 3%C stacking gel -the middle layer: 10%T 3%C spacer gel (1cm) -the lower layer: 16%T 6%C separating gel -voltage: 60V until the...

18 June 2019 9,796 1 View